Development of Hollow-Fiber Liquid-Phase Microextraction Method for Determination of Urinary trans, trans-Muconic Acid as a Biomarker of Benzene Exposure

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Development of Hollow-Fiber Liquid-Phase Microextraction Method for Determination of Urinary trans,trans-Muconic Acid as a Biomarker of Benzene Exposure

For the first time, hollow-fiber liquid-phase microextraction combined with high-performance liquid chromatography-ultraviolet was used to extract trans,trans-muconic acid, in urine samples of workers who had been exposed to benzene. The parameters affecting the metabolite extraction were optimized as follows: the volume of sample solution was 11 mL with pH 2, liquid membrane containing dihexyl...

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[Assessment of urinary trans, trans-muconic acid as a biomarker of exposure to benzene].

OBJECTIVE To assess the use of trans, trans-muconic acid as a biomarker of occupational exposure to benzene. METHODS Trans, trans-muconic acid in urine samples of exposed (exposed group, n=36) and non-exposed (non-exposed group, n=116) workers to benzene. Urinary levels of trans, trans-muconic acid were quantified by high-performance liquid chromatography. The study sample consisted of subjec...

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Urinary trans,trans-muconic acid as an indicator of exposure to benzene in cigarette smokers.

Epidemiological studies have shown an association between cigarette smoking and increased risk of myeloid leukemia in smokers. In evaluating this link it is important to note that cigarette smoke contains benzene, among other carcinogens. Since chronic benzene, among other carcinogens. Since chronic benzene exposure causes acute myeloid leukemia in humans, we aimed to determine the uptake and m...

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Urinary trans,trans-Muconic Acid as an Indicator of Exposure to Benzene in Cigarette Smokers1

Epidemiological studies have shown an association between cigarette smoking and increased risk of myeloid leukemia in smokers. In evaluating this link it is important to note that cigarette smoke contains benzene, among other carcinogens. Since chronic benzene exposure causes acute myeloid leukemia in humans, we aimed to determine the uptake and metabolic activation of benzene from cigarette sm...

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High-performance liquid chromatography method for urinary trans,trans-muconic acid. Application to environmental exposure to benzene.

This report describes a specific and precise high-performance liquid chromatography (HPLC) method for the quantification of trans,trans-muconic acid in human urine. The procedure involved a highly efficient Bond-Elut SAX extraction with 20% acetic acid elution. The HPLC analysis used a sodium acetate/methanol mobile phase with a C18 reverse phase column and UV detection at 265 nm. The recovery,...

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ژورنال

عنوان ژورنال: Analytical Chemistry Insights

سال: 2016

ISSN: 1177-3901,1177-3901

DOI: 10.4137/aci.s40177